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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was located to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two supplemental splice variants have been reported but in depth nucleotide sequence details is just not however readily available.
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Blocking peptides are peptides that bind especially to the target antibody and block antibody binding. These peptide typically consists of the epitope identified from the antibody.
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This guidebook outlines even more doable optimization of cellular and immunoassay parameters to be sure the very best success are...
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Antibodies bound to the blocking peptide no more bind into the epitope about the concentrate on protein. By evaluating the staining in the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Goal Information and facts Cyclin E belongs for the really conserved cyclin family members, whose users are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation patterns which contribute on the temporal coordination of every mitotic event. Cyclin E forms a fancy with and capabilities as a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates at the G1-S phase boundary which is degraded as cells progress by way of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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